Selectively infective phage (SIP) technology: scope and limitations.
نویسندگان
چکیده
We review here the selectively infective phage (SIP) technology, a powerful tool for the rapid selection of protein-ligand and peptide-ligand pairs with very high affinities. SIP is highly suitable for discriminating between molecules with subtle stability and folding differences. We discuss the preferred types of applications for this technology and some pitfalls inherent in the in vivo SIP method that have become apparent in its application with highly randomized libraries, as well as some precautions that should be taken in successfully applying this technology.
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ورودعنوان ژورنال:
- Journal of immunological methods
دوره 231 1-2 شماره
صفحات -
تاریخ انتشار 1999